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corneal epithelial cell growth kit  (ATCC)


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    ATCC corneal epithelial cell growth kit
    Corneal Epithelial Cell Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/corneal epithelial cell growth kit/product/ATCC
    Average 94 stars, based on 60 article reviews
    corneal epithelial cell growth kit - by Bioz Stars, 2026-03
    94/100 stars

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    Increased Prussian blue iron staining in human diabetic donor corneal biopsy tissue. ( A ) Representative images of whole mount preparations of human corneal biopsy punches from 69-year-old nondiabetic (NDM) and 64-year-old diabetic (DM) human donors. These punches were stained with Prussian blue and eosin. Scale bar , 200 µm. ( B ) Representative corneal epithelial images used for the semiquantitative iron staining score analysis. Scale bar , 50 µm. ( C ) Increased Prussian blue iron staining in human diabetic donor corneal epithelium ( n = 5). All values are mean ± SD.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ferroptosis: A Novel Mechanism in Diabetic Keratopathy

    doi: 10.1167/iovs.67.2.41

    Figure Lengend Snippet: Increased Prussian blue iron staining in human diabetic donor corneal biopsy tissue. ( A ) Representative images of whole mount preparations of human corneal biopsy punches from 69-year-old nondiabetic (NDM) and 64-year-old diabetic (DM) human donors. These punches were stained with Prussian blue and eosin. Scale bar , 200 µm. ( B ) Representative corneal epithelial images used for the semiquantitative iron staining score analysis. Scale bar , 50 µm. ( C ) Increased Prussian blue iron staining in human diabetic donor corneal epithelium ( n = 5). All values are mean ± SD.

    Article Snippet: HCEC were cultured in Corneal Epithelial Cell Basal Medium (PCS-700-030; ATCC) with Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC), following the manufacturer's recommended protocol.

    Techniques: Staining

    Iron accumulation and TfR1 upregulation in STZ-diabetic (STZ-DM) mouse corneas. ( A ) Representative Prussian blue staining images showing iron staining in paraffin sections from STZ-DM mouse corneas. The arrow indicates the location of the iron staining in the cornea. ( B ) STZ-DM mice exhibit a significant reduction in corneal epithelial thickness compared with nondiabetic (NDM) controls ( n = 5). ( C ) Representative corneal flat mounts image (with epithelium side up) showing Prussian blue staining for iron in the corneas of NDM and STZ-DM mice. The arrows indicate the location of the iron staining in the cornea. ( D ) Increased iron staining scores in corneal flat mounts from STZ-DM mice ( n = 5). ( E , F ) Western blot showing increased TfR1 expression in STZ-DM mouse corneas ( n = 5). All values are mean ± SD.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Ferroptosis: A Novel Mechanism in Diabetic Keratopathy

    doi: 10.1167/iovs.67.2.41

    Figure Lengend Snippet: Iron accumulation and TfR1 upregulation in STZ-diabetic (STZ-DM) mouse corneas. ( A ) Representative Prussian blue staining images showing iron staining in paraffin sections from STZ-DM mouse corneas. The arrow indicates the location of the iron staining in the cornea. ( B ) STZ-DM mice exhibit a significant reduction in corneal epithelial thickness compared with nondiabetic (NDM) controls ( n = 5). ( C ) Representative corneal flat mounts image (with epithelium side up) showing Prussian blue staining for iron in the corneas of NDM and STZ-DM mice. The arrows indicate the location of the iron staining in the cornea. ( D ) Increased iron staining scores in corneal flat mounts from STZ-DM mice ( n = 5). ( E , F ) Western blot showing increased TfR1 expression in STZ-DM mouse corneas ( n = 5). All values are mean ± SD.

    Article Snippet: HCEC were cultured in Corneal Epithelial Cell Basal Medium (PCS-700-030; ATCC) with Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC), following the manufacturer's recommended protocol.

    Techniques: Staining, Western Blot, Expressing